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CiDao®‌ Ceramic Hydroxyapatite for Chromatography CD-CHT
Product Introduction

Hydroxyapatite, as an inorganic compound, is widely used in many scientific fields due to its unique chemical structure - Ca10(PO4)6(OH)2. As a chromatographic resin, ceramic hydroxyapatite combines ion exchange and metal affinity mechanisms, thus possessing a special separation capacity unmatched by other traditional materials, making it one of the currently highly anticipated mixed-mode chromatographic resins. Leveraging the cutting-edge R&D expertise and manufacturing processes, Pinshan Biotech has successfully innovated a stronger, more porous hydroxyapatite material — Ceramic Hydroxyapatite for Chromatography (abbreviated as CD-CHT). This material is a spherical, macroporous form of hydroxyapatite and is crafted by agglomerating and sintering the crystals at high temperatures, resulting in robust spherical, macroporous beads. CD-CHT exhibits remarkable durability, withstanding very high flow rates and pressures, making it ideal for the separation and purification of a wide range of biological molecules.

Product Highlights
Independent Intellectual Property Rights
FDA DMF Filing Successfully
Multiple Particle Sizes Available for Selection
Extensive Range of Applications
High Purity Materials
High Capacity Characteristics
High Reliability Assurance
Stable Supply
Market Applications
Different subclasses of monoclonal and polyclonal antibodies
Antibodies that differ in light chain composition
Antibody fragments
Vaccines
Blood products
Recombinant proteins
Viral particles
Supercoiled DNA from linear duplexes
Single-stranded and double-stranded DNA
Isozymes
Technical Parameters

To meet the diverse needs of biological separation and purification, Pinshan Biotech has developed two types of ceramic hydroxyapatite (Type Ⅰ and Type Ⅱ). Additionally, three particle sizes (20μm, 40μm, 60μm) are provided for selection, ensuring that the products can adapt to different application needs of customers.

Ceramic Hydroxyapatite Type Ⅰ Resin:
Fully sintered at 400°C, forming uniform spherical particles.
With a high protein binding capacity, it is particularly suitable for the efficient separation of acidic proteins.
Ceramic Hydroxyapatite Type Ⅱ Resin:
Fully sintered at 700°C, it also forms uniform spherical particles.
Compared to Type Ⅰ, it has a lower protein binding capacity but excels in resolving early elution proteins and nucleic acids.
With lower affinity for albumin, it is more suitable for purifying various types of immunoglobulins.
Type Ⅰ Type Ⅱ
Functional Group Ca2+, PO43-, OH-
Particle Size 20µm, 40µm, 60µm
Recommended Operating Flow Rate 50-1000 cm/h.
pH Stability 6.5-14
Chemical Compatibility (> 24 hr.) 1 M NaOH, 8 M urea, 8 M guanidine hydrochloride, ethanol, methanol, 100% acetonitrile
Regeneration 0.4-1.0 M phosphate buffer, 0.5 M sodium phosphate, PH 7.0; 1.0 M trisodium phosphate, pH 11–12
Sanitization 1–2 M NaOH
Autoclavability (121ºC, 30 min)
Tap-settled Density (g/ml Tap-settled Bed) 0.6-0.7g/mL
Dynamic Capacity Egg white lysozyme > 25 mg/ml Egg white lysozyme > 12.5 mg/ml
Standard Pore Diameter 600–800Å 800–1000Å
Maximum Operating Pressure 10Mpa 10Mpa
Ordering Information
Product Name Type Particle Size Net Wt.
CD = Ceramic Hydroxyapatite 101=Type I A=20μm
B=40μm
C=60μm
01=25g/Bottle
02=100g/Bottle
03=250g/Bottle
102=Type II 04=500g/Bottle
05=1kg/Bottle
06=5kg/Bucket
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