Affinity Chromatography (AC) is a chromatography technique that utilizes specific biological affinity for molecular separation. When the sample passes through the stationary phase, target biomolecules bind specifically to the ligand and remain on the stationary phase, while impurities unable to bind elute with the buffer, achieving biomolecule purification. This technique is one of the most commonly used chromatography methods for protein and enzyme purification, offering simple and rapid separation processes, and is also frequently applied in structural/functional studies of biomolecules.
Protein G Singarose 4 FF is prepared by coupling recombinant Protein G to hydrogen bromide-activated Singarose 4 FF resin. The Protein G ligand is produced via recombinant E. coli expression with an animal-free manufacturing process. This ligand specifically binds to the Fc region of IgG and exhibits weak interaction with Fab regions, making it ideal for purifying immunoglobulins from serum, ascites, tissue culture supernatants, and other sources.