Products
lgM / IgY Capture Resin
Product Introduction
Affinity Chromatography (AC) utilizes specific biological affinity for molecular separation. When samples pass through the stationary phase, target biomolecules bind specifically to the ligand while impurities elute with the buffer, achieving biomolecule purification. This technique is one of the most commonly used chromatography methods for protein/enzyme purification, offering simple and rapid separation processes, and is frequently applied in structural/functional studies of biomolecules.
IgM Capture Singarose HP / IgY Capture Singarose HP are thiophilic affinity chromatography resins prepared by coupling 2-mercaptopyridine (a sulfur-containing compound) to highly cross-linked 6% agarose resin. With an average particle size of 34 μm, these resins exhibit high resolution. The thiophilic interaction, enhanced under high-salt conditions and attenuated in low-salt environments, enables selective biomolecule separation.
IgM Capture Singarose HP is primarily used for rapid and efficient purification of IgM. IgY Capture Singarose HP specializes in extracting IgY from egg yolk and can also purify other immunoglobulins.
Product Highlights
High Resolution, High Binding Capacity
Excellent Stability, Good Reproducibility
Strong Hydrophilicity, Minimal Non-specific Adsorption
Technical Parameters
Product Name IgM Capture Singarose HP IgY Capture Singarose HP
Matrix 6% Highly Cross-linked Agarose Resin
Average Particle Size 34 µm
Ligand 2-Mercaptopyridine
Binding Capacity >5 mg IgM/mL Resin >20 mg IgY/mL Resin
Recommended Flow Rate <150 cm/h
Recommended Pressure <0.3 MPa (3 bar)
pH Stability 3-11 (Long-term*); 2-13 (Short-term*)
Chemical Stability Stable in common aqueous buffers, including 70% Ethanol, 0.1 M NaOH, 1 M Acetic Acid
Storage Conditions 20% Ethanol; 4-30℃

Note *: Long-term refers to the pH range where the resin maintains stability without compromising performance. Short-term refers to pH ranges experienced during regeneration, CIP, and sanitization based on empirical data.

Ordering Information
Product Name Catalog No. Specification
IgM Capture Singarose HP HC1110001 25 mL
HC1110002 100 mL
HC1110004 1 L
HC1110005 5 L
IgY Capture Singarose HP HC1120001 25 mL
HC1120002 100 mL
HC1120004 1 L
HC1120005 5 L
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