Metal Chelation Affinity Chromatography, also known as Immobilized Metal Ion Affinity Chromatography (IMAC), utilizes specific interactions between amino acid residues (histidine, tryptophan, cysteine) on protein surfaces and metal ions (Ni²⁺, Cu²⁺, Zn²⁺, Co²⁺, Ca²⁺, Fe³⁺) for protein purification.

Aplo Ni Singarose FF is a novel IMAC resin that leverages histidine-mediated metal ion affinity for biomolecule separation. Nickel ions are stably chelated onto a high-flow agarose matrix, offering excellent specificity, rapid flow rates, and minimal metal leakage. This enables efficient target protein purification even in EDTA/DTT-containing buffers, with superior batch-to-batch reproducibility and uniform particle size. Applications include purification of histidine-containing peptides, proteins, nucleotides, phosphorylated proteins, and His-tagged recombinant proteins.

Ni Singarose FF/HP are IMAC resins prepared by coupling nitrilotriacetic acid (NTA) to a 6% agarose matrix followed by Ni²⁺ chelation. Key advantages include high dynamic binding capacity, low Ni²⁺ ligand leakage, exceptional selectivity, and tolerance to reducing agents. These resins are widely used for downstream purification of proteins/peptides in biopharmaceuticals and bioengineering, particularly for efficient His-tagged protein purification.