Metal Chelation Affinity Chromatography, also known as Immobilized Metal Ion Affinity Chromatography (IMAC), utilizes specific interactions between amino acid residues (histidine, tryptophan, cysteine) on protein surfaces and metal ions (Ni²⁺, Cu²⁺, Zn²⁺, Co²⁺, Ca²⁺, Fe³⁺) for protein purification.
IMAC Singarose FF/HP are metal chelation affinity resins prepared by coupling nitrilotriacetic acid (NTA) ligands to a 6% highly cross-linked agarose matrix. These resins provide four coordination sites for stable metal ion chelation (Ni²⁺/Cu²⁺/Zn²⁺/Co²⁺) and exhibit excellent tolerance to reducing agents. After user-specific metal ion loading, they are suitable for downstream purification of proteins/peptides in biopharmaceuticals and bioengineering applications.
