Metal Chelation Affinity Chromatography, also known as Immobilized Metal Ion Affinity Chromatography (IMAC), utilizes specific interactions between amino acid residues (histidine, tryptophan, cysteine) on protein surfaces and metal ions (Ni²⁺, Cu²⁺, Zn²⁺, Co²⁺, Ca²⁺, Fe³⁺) for protein purification.
Chelating Singarose FF is a metal chelation affinity resin prepared by coupling iminodiacetic acid (IDA) ligands to a 6% highly cross-linked agarose matrix. This resin provides three coordination sites for metal ion chelation (Ni²⁺/Cu²⁺/Zn²⁺/Co²⁺) and another three coordination sites for protein binding via the chelated metal ions, achieving high binding capacity. After user-specific metal ion loading, it is suitable for downstream purification of proteins/peptides in biopharmaceutical and bioengineering applications.
