GST Tag Affinity Chromatography Resin Glutathione Singarose 4 FF Glutathione Singarose 4B Glutathione Singarose HP-Pinshan Biotech - A Supplier of Core Consumables and Solutions for Biopharmaceuticals-Pinshan Biotechnology (Shanghai) Co., Ltd

GST Resin

Product Introduction

Affinity Chromatography (AC) is a separation technique utilizing specific biological interactions. When sample passes through the stationary phase, target biomolecules bind to ligands while impurities elute, achieving purification. AC is widely used for proteins, enzymes, and other macromolecules due to its simplicity, speed, and utility in structural/functional studies.

The GST (Glutathione S-transferase) tag is a common fusion partner in genetic engineering, enhancing soluble expression and activity retention of target proteins. Glutathione Singarose is an affinity resin prepared by coupling glutathione ligands to an agarose matrix, specifically designed for purifying GST and GST-fusion proteins with single-step high-purity elution.

Sample Application

Product Highlights

High Flow Rate, High Binding Capacity

Excellent Stability, Good Reproducibility

Strong Hydrophilicity, Minimal Non-specific Adsorption

Technical Parameters

Product Name	Glutathione Singarose 4 FF	Glutathione Singarose 4B	Glutathione Singarose HP
Matrix	4% Highly Cross-linked Agarose	4% Agarose	6% Highly Cross-linked Agarose
Average Particle Size	90 µm		34 µm
Ligand	Glutathione with 10-atom spacer arm
Binding Capacity	>10 mg GST-fusion Protein/mL Resin	>25 mg Horse Liver Glutathione/mL Resin	>10 mg GST-fusion Protein/mL Resin
Recommended Flow Rate	＜250 cm/h	＜75 cm/h	＜150 cm/h
Recommended Pressure	＜0.15 MPa (1.5 bar)	＜0.01 MPa (0.1 bar)	＜0.3 MPa (3 bar)
pH Stability	3-12 (Long-term); 3-12 (Short-term)	4-13 (Long-term); 4-13 (Short-term)	3-12 (Long-term); 3-12 (Short-term)
Chemical Compatibility	Stable in common buffers including 0.1 M NaOH, 1 M Acetic Acid, 6 M Guanidine Hydrochloride, 70% Ethanol
Storage Conditions	20% Ethanol; 4-30℃

Note *: Long-term refers to resin stability without performance degradation. Short-term refers to pH ranges experienced during regeneration, CIP, and sanitization.

Ordering Information

Product Name	Catalog No.	Specification
Glutathione Singarose 4 FF	HC1030001	25 mL
	HC1030002	100 mL
	HC1030004	1 L
	HC1030005	5 L
Glutathione Singarose 4B	HC1030071	25 mL
	HC1030072	100 mL
	HC1030074	1 L
	HC1030075	5 L
Glutathione Singarose HP	HC1040001	25 mL
	HC1040002	100 mL
	HC1040004	1 L
	HC1040005	5 L

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