Strong Cation Exchange Chromatography Resin (SP) Captarose HF SP Singarose FF SP Singarose HP SP Singarose XL SP Singarose BB-Pinshan Biotech - A Supplier of Core Consumables and Solutions for Biopharmaceuticals-Pinshan Biotechnology (Shanghai) Co., Ltd

Strong Cation Exchange Resin (SP)

Product Introduction

Ion Exchange Chromatography (IEC) utilizes ion exchange ligands as the stationary phase, enabling separation based on the reversible exchange of ions between mobile phase components and stationary phase counter-ions, leveraging differences in binding affinity. This method offers high resolution, large working capacity, and operational simplicity, making it one of the most frequently used purification technologies for biopharmaceuticals, proteins, polypeptides, and other biomolecules. The IEC resin structure comprises three essential components: Cross-linked polymeric matrix (base matrix); Functional groups covalently attached to the matrix; Mobile ions carrying opposite charges to the functional groups, capable of undergoing ion exchange.

Sample Application

Product Series

Captarose HF series features a highly rigid agarose microsphere matrix with an average particle size of 75 μm. The Captarose matrix undergoes chemical modification and engineering to achieve superior mechanical properties, delivering high dynamic binding capacity under elevated flow rates for rapid processing of large sample volumes.

Captarose HR series utilizes a highly rigid agarose microsphere matrix (40 μm average particle size) derived from Singarose HP through chemical modification. It maintains excellent mechanical integrity while achieving superior resolution under elevated flow rates.

Singarose FF series employs a 6% highly cross-linked agarose microsphere matrix (90 μm average particle size) with functional groups covalently immobilized via stable bonds. Characterized by high flow rates and broad applicability, it serves as a standard resin for large-scale purification of biomacromolecules.

Singarose HP series features a 6% highly cross-linked agarose microsphere matrix (34 μm average particle size) with functional groups covalently attached. Known for high resolution, it is widely used in intermediate and polishing steps of downstream processing.

Singarose XL series incorporates a 6% highly cross-linked agarose microsphere matrix (90 μm average particle size) with extended dextran chains. The protruded dextran structure enhances ligand exposure, resulting in higher flow rates, increased binding capacity, and improved charge-based protein adsorption.

Singarose BB series utilizes a 6% highly cross-linked agarose microsphere matrix (200 μm average particle size). Its large particle size enables ultra-high flow rates and void volume, making it ideal for processing large volumes of crude extracts or viscous samples in capture steps of downstream purification.

Technical Parameters

Product Name	SP Captarose HF	SP Captarose HR	SP Singarose FF	SP Singarose HP	SP Singarose XL	SP Singarose BB	SP Singarose XL BB
Matrix	Highly rigid agarose		6% highly cross-linked agarose		6% highly cross-linked agarose with dextran branches	6% highly cross-linked agarose	6% highly cross-linked agarose with dextran branches
Average Particle Size	75 µm	40 µm	90 µm	34 µm	90 µm	200 µm
Ligand	Sulfopropyl groups
Ion Loading Capacity	130-160 μmol H⁺/mL resin		180-250 μmol H⁺/mL resin	150-200 μmol H⁺/mL resin	180-250 μmol H⁺/mL resin	180-250 μmol H⁺/mL resin	230-330 μmol H⁺/mL resin
Recommended Flow Rate	＜600 cm/h	＜300 cm/h	＜400 cm/h	＜150 cm/h	＜400 cm/h	＜1800 cm/h
Recommended Pressure	＜0.5 MPa (5 bar)		＜0.3 MPa (3 bar)
Operating pH Range*	4-13
pH Stability	4-13 (Long-term); 3-14 (Short-term)
Chemical Stability	Stable in common buffers including 1 M NaCl, 1 M NaOH, 6 M guanidine hydrochloride, 8 M urea, 70% ethanol, 30% isopropanol
Storage Conditions	20% ethanol containing 0.2 M sodium acetate

Notes *: Operating pH range refers to the pH conditions under which the resin maintains separation performance while remaining stable.
Notes **: Long-term refers to the pH range where the resin maintains stability without adverse effects on subsequent performance. Short-term refers to the pH range empirically established for resin regeneration, cleaning-in-place (CIP), and sanitization.

Ordering Information

Product Name	Catalog No.	Specification
SP Captarose HF	HC2040001	25 mL
	HC2040002	100 mL
	HC2040004	1 L
	HC2040005	5 L
SP Captarose HR	HC2040101	25 mL
	HC2040102	100 mL
	HC2040104	1 L
	HC2040105	5 L
SP Singarose FF	HC2040201	25 mL
	HC2040202	100 mL
	HC2040204	1 L
	HC2040205	5 L
SP Singarose HP	HC2040301	25 mL
	HC2040302	100 mL
	HC2040304	1 L
	HC2040305	5 L
SP Singarose XL	HC2040401	25 mL
	HC2040402	100 mL
	HC2040404	1 L
	HC2040405	5 L
SP Singarose BB	HC2040501	25 mL
	HC2040502	100 mL
	HC2040504	1 L
	HC2040505	5 L
SP Singarose XL BB	HC2040601	25 mL
	HC2040602	100 mL
	HC2040604	1 L
	HC2040605	5 L

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