Weak Anion Exchange Chromatography Resin (DEAE) Captarose HF DEAE Singarose FF DEAE Singarose HP-Pinshan Biotech - A Supplier of Core Consumables and Solutions for Biopharmaceuticals-Pinshan Biotechnology (Shanghai) Co., Ltd

Weak Anion Exchange Resin (DEAE)

Product Introduction

Ion Exchange Chromatography (IEC) utilizes ion exchange ligands as the stationary phase, enabling separation based on the reversible exchange of ions between mobile phase components and stationary phase counter-ions, leveraging differences in binding affinity. This method offers high resolution, large working capacity, and operational simplicity, making it one of the most frequently used purification technologies for biopharmaceuticals, proteins, polypeptides, and other biomolecules. The IEC resin structure comprises three essential components: Cross-linked polymeric matrix (base matrix); Functional groups covalently attached to the matrix; Mobile ions carrying opposite charges to the functional groups, capable of undergoing ion exchange.

Sample Application

Product Series

Captarose HF series features a highly rigid agarose microsphere matrix with an average particle size of 75 μm. The Captarose matrix undergoes chemical modification and engineering to achieve superior mechanical properties, delivering high dynamic binding capacity under elevated flow rates for rapid processing of large sample volumes.

Singarose FF series employs a 6% highly cross-linked agarose microsphere matrix (90 μm average particle size) with functional groups covalently immobilized via stable bonds. Characterized by high flow rates and broad applicability, it serves as a standard resin for large-scale purification of biomacromolecules.

Singarose HP series features a 6% highly cross-linked agarose microsphere matrix (34 μm average particle size) with functional groups covalently attached. Known for high resolution, it is widely used in intermediate and polishing steps of downstream processing.

Technical Parameters

Product Name	DEAE Captarose HF	DEAE Singarose FF	DEAE Singarose HP
Matrix	Highly rigid agarose	6% highly cross-linked agarose
Average Particle Size	75 µm	90 µm	34 µm
Ligand	Diethylaminoethyl groups
Ion Loading Capacity	290-350 μmol Cl⁻/mL resin	110-160 μmol Cl⁻/mL resin
Recommended Flow Rate	＜600 cm/h	＜400 cm/h	＜150 cm/h
Recommended Pressure	＜0.5 MPa (5 bar)	＜0.3 MPa (3 bar)
Operating pH Range*	2-9
pH Stability	2-12 (Long-term); 2-14 (Short-term)	3-12 (Long-term); 1-14 (Short-term)
Chemical Stability	Stable in common buffers including 1 M NaCl, 1 M NaOH, 6 M guanidine hydrochloride, 8 M urea, 70% ethanol, 30% isopropanol
Storage Conditions	20% ethanol

Notes *: Operating pH range refers to the pH conditions under which the resin maintains separation performance while remaining stable.
Notes **: Long-term refers to the pH range where the resin maintains stability without adverse effects on subsequent performance. Short-term refers to the pH range empirically established for resin regeneration, cleaning-in-place (CIP), and sanitization.

Ordering Information

Product Name	Catalog No.	Specification
DEAE Captarose HF	HC2010001	25 mL
	HC2010002	100 mL
	HC2010004	1 L
	HC2010005	5 L
DEAE Singarose FF	HC2010201	25 mL
	HC2010202	100 mL
	HC2010204	1 L
	HC2010205	5 L
DEAE Singarose HP	HC2010301	25 mL
	HC2010302	100 mL
	HC2010304	1 L
	HC2010305	5 L

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