Products
Singarose FF Resin (HIC)
Product Introduction
Singarose FF Hydrophobic Interaction Chromatography (HIC) Resin utilizes highly cross-linked agarose microspheres as the matrix, with an average particle size of 90 μm. Functional groups are covalently immobilized onto the agarose microspheres through stable chemical bonds. The charge-free ligands enable true hydrophobic interaction chromatography without ionic interference. Characterized by fast flow rates and broad applicability, this resin is a preferred choice for large-scale separation and purification of biomolecules. With superior kinetic performance, Singarose FF HIC Resin is particularly suitable for initial capture and intermediate purification steps in purification processes.
Product Highlights
Uniform Particle Size
High Flow Rate
Excellent Stability
Superior Reproducibility
Scalable
Strong Hydrophilicity, Minimal Non-specific Adsorption
High Versatility, Broad Application Range
Technical Parameters
Product Name Phenyl Singarose FF (HS) Phenyl Singarose FF (LS) Butyl-S Singarose FF Butyl Singarose 4 FF Octyl Singarose 4 FF
Matrix 6% Highly Cross-linked Agarose 4% Highly Cross-linked Agarose
Average Particle Size 90 µm
Ligand Phenyl Butyl-S Butyl Octyl
Ligand Density 45 µmol/mL resin 25 µmol/mL resin 10 µmol/mL resin 40 µmol/mL resin 5 µmol/mL resin
Recommended Flow Rate <400 cm/h <250 cm/h
Recommended Pressure <0.3 MPa (3 bar) <0.15 MPa (1.5 bar)
pH Stability 3-13 (Long-term*); 2-14 (Short-term*)
Chemical Stability Stable in common aqueous buffers including: 1 mM HCl, 1 M NaOH, 30% Isopropanol, 6 M Guanidine Hydrochloride, 8 M Urea, etc.
Operating Temperature 4-40℃
Storage Conditions 20% Ethanol; 4-30℃

Note *: Long-term refers to pH range where the resin maintains stability without compromising performance. Short-term refers to pH range for regeneration, in-place cleaning, and sterilization.

Ordering Information
Product Name Catalog No. Specification
Phenyl Singarose FF (HS) HC4030101 25 mL
HC4030102 100 mL
HC4030104 1 L
HC4030105 5 L
Phenyl Singarose FF (LS) HC4030201 25 mL
HC4030202 100 mL
HC4030204 1 L
HC4030205 5 L
Butyl-S Singarose FF HC4040101 25 mL
HC4040102 100 mL
HC4040104 1 L
HC4040105 5 L
Butyl Singarose 4 FF HC4010101 25 mL
HC4010102 100 mL
HC4010104 1 L
HC4010105 5 L
Octyl Singarose 4 FF HC4020101 25 mL
HC4020102 100 mL
HC4020104 1 L
HC4020105 5 L
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