Hydroxyapatite, as an inorganic compound, is widely used in many scientific fields due to its unique chemical structure - Ca10(PO4)6(OH)2. It possesses excellent biocompatibility and bioactivity, making it a promising material with extensive applications in the biopharmaceutical field.
Binding Mechanism
Ceramic hydroxyapatite stands apart from traditional chromatographic resins due to its dual-site structure—Ca2+ and PO43-—which combines multiple protein binding mechanisms, including anion exchange, cation exchange, and metal affinity. Specifically, the PO43- site forms ionic bonds with positively charged proteins, demonstrating cation exchange traits, whereas the Ca2+ site interacts with negatively charged proteins via metal affinity or anion exchange with available carboxyl clusters. These two distinct mechanisms have positioned hydroxyapatite as a vital chromatographic resin in biopharmaceutical downstream processing, attracting growing interests in the production of antibodies, vaccines, nucleic acids, enzymes, and recombinant proteins.
Product Characteristics
Pinshan Biotech's ceramic hydroxyapatite chromatographic resin, CD-CHT, is carefully crafted through high-temperature sintering, resulting in a stable structure and uniform particle size, making it the preferred chromatographic resin for efficient separation and purification.
❶ High Quality Standards:
● GMP standard production
● ISO9001 quality system certification
● FDA DMF filing successfully obtained
❷ Dual Selectivity：Have unique separation properties and unparalleled selectivity and resolution, distinct from traditional modes. Can meet specific separation needs.
❸ Efficient Separation：Exhibit excellent separation efficiency for biologically similar and difficult-to-separate molecules, breaking through the limitations of traditional resins.
❹ Wide Applications：
● Different subclasses of monoclonal and polyclonal antibodies
● Antibodies that differ in light chain composition
● Antibody fragments
● Recombinant proteins
● Viral particles
● Vaccines
● Supercoiled DNA from linear duplexes
● Single-stranded from double-stranded DNA
● Isozymes
Specifications
To meet the diverse needs of biological separation and purification, Pinshan Biotech has developed two types of ceramic hydroxyapatite (Type Ⅰ and Type Ⅱ). Additionally, three particle sizes (20μm, 40μm, 60μm) are provided for selection, ensuring that the products can adapt to different application needs of customers.
◆Ceramic Hydroxyapatite Type Ⅰ Resin：
Fully sintered at 400°C, forming uniform spherical particles.
With a high protein loading capacity, it is particularly suitable for the efficient separation of acidic proteins.
◆ Ceramic Hydroxyapatite Type Ⅱ Resin：
Fully sintered at 700°C, it also forms uniform spherical particles.
Compared to Type Ⅰ, it has a lower protein loading capacity but excels in resolving early elution proteins and nucleic acids.
With lower affinity for albumin, it is more suitable for purifying various types of immunoglobulins.
Performance Comparison
When compared to other brands available on the market, Pinshan Biotech's CD-CHT stands out not only for its superior performance but also for its dependable and consistent supply support backed by professional technical assistance. This comprehensive package ensures a solid foundation for your experiments and production, providing you with a robust guarantee.
*When separating antibody samples, the yield of the recovered product is superior to other brands, with consistent separation performance.
*Pinshan Biotech's CD-CHT exhibits a back pressure of only 0.71Mp at a linear flow rate of up to 4586cm/hr, while the imported product has a back pressure of 1.58Mp at the same flow rate.
Technical Parameters
Ordering Information
